Role of PAL and PPO Enzyme Activity in Host Pathogen interaction of Chickpea (Cicer Arietinum L) Root Tissue Infected with Fusarium Wilt

DOI: 10.22161/ijeab/1.4.41

Abstract:
In this study, changes in the activities of Phenlylalanine ammonialyase (PAL) and polyphenol oxidase (PPO) during development and stages of wilt (Fusarium oxysporum Schlechtend.:Fr. f.sp. ciceri (Padwick) Matuo & K. Sato) disease infection in chickpea(Cicer arietinum L) were investigated. During the early stages of disease development, at pre-infectional stage (S1), cultivars did not show any significant change in PAL activity. The activity was significantly increased at infectional stage (S2) as compared with pre infectional stage (S1). Susceptible cultivars had the lower value of PAL at infectional stage (S2). However, at S2 stage cultivars GG-4 and JCP-27 were at par. At post infectional stage (S3), the activity was found to be increased in the all cultivars as compared to infectional or mid growth stage and the resistant and tolerant cultivars were at par. Susceptible cultivars (JG-62 and GG-4) had the lower activity as compared to the resistant and tolerant cultivars. The resistant cultivars WR-315 and JCP-27 revealed higher level of activity, the level of activity significantly increased marginally during infection. In the present experiment significantly higher activity in infected plants grown in sick plot also suggested that polyphenol oxidase might be involved in oxidation of phenolics in susceptible cultivar (JG-62). These observations suggest that the increase in PAL and PPO activities has an important role in disease resistance mechanism.

Keywords:
chickpea, Polyphenols oxidase (PPO), phenylalanine ammonia lyase(PAL).

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